Hoelting T, Duh Q Y, Clark O H, Herfarth C
Chirurgische Universitätskliniken Heidelberg.
Langenbecks Arch Chir Suppl Kongressbd. 1998;115(Suppl I):281-4.
TSH is the classic stimulator of thyroid cell function. Clinically, treatment with thyroxin to suppress TSH decreased the risk of thyroid cancer recurrence and improved patient survival. This study analyzed the effect of stably transfected human TSH receptor cDNA in an established model of metastatic follicular thyroid cancer cells (FC) compared to wild type FTC. Wild type FTC lack TSH receptors and do not depend on TSH for growth. However, they contain thyroglobulin, have intact thyroid functions and response to TSH. We tested growth, invasion, and adhesion of transfected tumor cells (FTC-TSHr) compared to parental cells. All transfected FTC-TSHr expressed TSHr mRNA. Compared to wild type cells invasion and growth of TSHr-transfected FTC were significantly inhibited (p < 0.001). All FTC adhered best to collagen IV and fibronectin. Compared to parental cells adhesion of unstimulated FTC-TSHr was significantly enhanced (p < 0.001). These in vitro data underline the important role of the human TSH receptor as the main regulator of thyroid growth and functions.
促甲状腺激素（TSH）是甲状腺细胞功能的经典刺激因子。临床上，用甲状腺素治疗以抑制TSH可降低甲状腺癌复发风险并提高患者生存率。本研究分析了与野生型滤泡性甲状腺癌细胞（FTC）相比，稳定转染人TSH受体cDNA在已建立的转移性滤泡性甲状腺癌细胞模型中的作用。野生型FTC缺乏TSH受体，其生长不依赖TSH。然而，它们含有甲状腺球蛋白，具有完整的甲状腺功能且对TSH有反应。我们测试了转染的肿瘤细胞（FTC-TSHr）与亲本细胞相比的生长、侵袭和黏附情况。所有转染的FTC-TSHr均表达TSHr mRNA。与野生型细胞相比，TSHr转染的FTC的侵袭和生长受到显著抑制（p < 0.001）。所有FTC对IV型胶原和纤连蛋白的黏附性最佳。与亲本细胞相比，未刺激的FTC-TSHr的黏附性显著增强（p < 0.001）。这些体外数据强调了人TSH受体作为甲状腺生长和功能主要调节因子的重要作用。
