Engler S, Thiel C, David K, Juhl H
Klinik für Allgemeine Chirurgie und Thoraxchirurgie, Christian Albrechts-Universität, D-24105 Kiel.
Langenbecks Arch Chir Suppl Kongressbd. 1998;115(Suppl I):285-8.
We developed a human neuroblastoma animal model in nude rats, which shows high analogy to clinical stage IV disease. 10(7) LAN-1 human neuroblastoma cells were injected in the aorta of nude rats (rnu/rnu). After 5 weeks 6/6 rats developed invasive tumors in the adrenal gland (0.5-3 cm). Micrometastases (1-2 mm) were found in the liver (3 of 6 rats) and the femur (4 of 6 rats). This observation corresponds to the typical clinical finding of advanced neuroblastoma in the adrenal gland and small diffuse liver/bone metastases. A proliferation index of 80% was found in adrenal gland tumors and micrometastases. However, the number of apoptotic cells was different (5% and 50%, respectively). This animal model in nude rats can be used to evaluate the potential of new immunotherapeutic concepts with cytotoxic antibodies and to study the development of metastases.
我们在裸鼠中建立了一种人类神经母细胞瘤动物模型，该模型与临床IV期疾病高度相似。将10(7)个LAN-1人类神经母细胞瘤细胞注射到裸鼠（rnu/rnu）的主动脉中。5周后，6/6的大鼠在肾上腺出现侵袭性肿瘤（0.5 - 3厘米）。在肝脏（6只大鼠中的3只）和股骨（6只大鼠中的4只）中发现了微转移灶（1 - 2毫米）。这一观察结果与晚期神经母细胞瘤在肾上腺以及小的弥漫性肝/骨转移的典型临床发现相符。在肾上腺肿瘤和微转移灶中发现增殖指数为80%。然而，凋亡细胞的数量不同（分别为5%和50%）。这种裸鼠动物模型可用于评估新型细胞毒性抗体免疫治疗概念的潜力，并研究转移的发生发展。
