在4摄氏度和零下2摄氏度下，鲤鱼（Cyprinus carpio L.）胚胎在不同浓度甲醇和蔗糖中的孵化情况。

Hatching of common carp (Cyprinus carpio L.) embryos stored at 4 and -2 degrees C in different concentrations of methanol and sucrose.

作者信息

Ahammad M M, Bhattacharyya D, Jana B B

机构信息

Aquaculture Research Unit, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.

出版信息

Theriogenology. 2003 Nov;60(8):1409-22. doi: 10.1016/s0093-691x(03)00118-3.

DOI:10.1016/s0093-691x(03)00118-3

PMID:14519463

Abstract

The hatching performance of common carp (Cyprinus carpio L.) embryos was examined after 12-72-h storage at 4 and -2 degrees C using different concentrations of sucrose (0.1, 0.25, 0.5 and 1.0 M or 3.42, 8.55, 17.10 and 34.2%), methanol (MeOH) (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 M or 1.6, 3.2, 4.8, 6.4, 8.0, 9.6 and 11.2%), or varying concentrations of methanol in 0.5 M (17.10%) sucrose. For sucrose, 0.5 M (17.10%) showed the maximum survival (41+/-1% (12 h) to 11+/-1.5% (72 h)) at 4 degrees C. No survival was observed at -2 degrees C with any concentration of sucrose. At both temperatures employed, hatching was higher with mixed combination of methanol (1.5 M or 4.8%) and 0.5 M (17.10%) sucrose (4 degrees C: 41+/-1.5% (12 h), 38+/-1.2% (72 h); -2 degrees C: 33+/-1.7% (12 h), 28+/-1.2% (72 h)) compared to methanol alone (4 degrees C: 38+/-1.5% (12 h), 35+/-2.5% (72 h); -2 degrees C: 31+/-2.5% (12 h), 25+/-2% (72 h)). The combination of 1.5 M (4.8%) methanol and 0.5 M (17.10%) sucrose produced the best results among all the concentrations tested at both temperatures.

摘要

使用不同浓度的蔗糖（0.1、0.25、0.5和1.0 M或3.42%、8.55%、17.10%和34.2%）、甲醇（MeOH）（0.5、1.0、1.5、2.0、2.5、3.0和3.5 M或1.6%、3.2%、4.8%、6.4%、8.0%、9.6%和11.2%）或0.5 M（17.10%）蔗糖中不同浓度的甲醇，在4℃和-2℃下储存12 - 72小时后，检测鲤鱼（Cyprinus carpio L.）胚胎的孵化性能。对于蔗糖，0.5 M（17.10%）在4℃时显示出最高的存活率（12小时时为41±1%，72小时时为11±1.5%）。在-2℃下，任何浓度的蔗糖都未观察到存活情况。在所用的两个温度下，与单独使用甲醇相比，甲醇（1.5 M或4.8%）和0.5 M（17.10%）蔗糖的混合组合孵化率更高（4℃：12小时时为41±1.5%，72小时时为38±1.2%；-2℃：12小时时为33±1.7%，72小时时为28±1.2%）（单独使用甲醇时，4℃：12小时时为38±1.5%，72小时时为35±2.5%；-2℃：12小时时为31±2.5%，72小时时为25±2%）。在两个温度下测试的所有浓度中，1.5 M（4.8%）甲醇和0.5 M（17.10%）蔗糖的组合产生了最佳结果。