马铃薯植株（茄属马铃薯种）中氘标记茉莉酸和OPC 8:0的代谢

Metabolism of deuterium-labeled jasmonic acid and OPC 8:0 in the potato plant (Solanum tuberosum L.).

作者信息

Matsuura Hideyuki, Yoshihara Teruhiko

机构信息

Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.

出版信息

Biosci Biotechnol Biochem. 2003 Sep;67(9):1903-7. doi: 10.1271/bbb.67.1903.

DOI:10.1271/bbb.67.1903

PMID:14519974

Abstract

The metabolism of deuterium-labeled (+/-)-jasmonicacid and 3-oxo-2-[(Z)pent-2'-enyl]-cyclopentan-1- octanoic acid in potato (Solanum tuberosum L.) was examined by using cultures of potato single-node stems. Deuterium-labeled (+/-)-jasmonic acid and 3-oxo-2-[(Z) pent-2'-enyl]cyclopentan-1-octanoic acid, which had been prepared from commercially available methyl (+/-)-jasmonate, were fed to the cultures, and the metabolites were extracted from the plants and analyzed by a liquid chromatography-selected ion monitoring system. The metabolism of deuterium-labeled (+/-)-jasmonic acid and 3-oxo-2-[(Z)-2'-pentenyl]cyclopentan-1-octanoic acid to 5' and 4'-O-glucopyranosyloxyjasmoic acids was strongly suggested.

摘要

利用马铃薯单节茎段培养物研究了氘标记的（±）-茉莉酸和3-氧代-2-[(Z)-戊-2'-烯基]-环戊烷-1-辛酸在马铃薯（Solanum tuberosum L.）中的代谢。将由市售（±）-茉莉酸甲酯制备的氘标记的（±）-茉莉酸和3-氧代-2-[(Z)-戊-2'-烯基]环戊烷-1-辛酸加入到培养物中，从植物中提取代谢产物，并通过液相色谱-选择离子监测系统进行分析。强烈提示氘标记的（±）-茉莉酸和3-氧代-2-[(Z)-2'-戊烯基]环戊烷-1-辛酸代谢生成5'-O-吡喃葡萄糖基氧基茉莉酸和4'-O-吡喃葡萄糖基氧基茉莉酸。