Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain DSS12.
作者信息
Kawano Hiroaki, Ikegami Akihiko, Nakasone Kaoru, Kato Chiaki, Usami Ron, Horikoshi Koki
机构信息
Department of Applied Chemistry, Faculty of Engineering, Toyo University, Kawagoe, Saitama, Japan.
出版信息
Biosci Biotechnol Biochem. 2003 Sep;67(9):1983-5. doi: 10.1271/bbb.67.1983.
PMID:14519985
Abstract
NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.
摘要
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