Shibata Hitoshi, Nagamine Takayuki, Wang Yong, Ishikawa Takahiro, Sawa Yoshihiro
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.
Biosci Biotechnol Biochem. 2003 Sep;67(9):1996-8. doi: 10.1271/bbb.67.1996.
Near-UV irradiation caused the decomposition of hinokitiol in an aqueous solution. During the photochemical reaction, the distinct electron spin resonance signal characteristic of the adduct of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) with the hydroxyl radical was accompanied by small signals corresponding to the adduct of DMPO with the superoxide anion radical. More than 95% of Escherichia coli cells were killed by the incubation with hinokitiol under near-UV irradiation by BLB fluorescent lamps. These results indicated the generation of reactive oxygen species during photochemical reaction of hinokitiol under near-UV irradiation.
近紫外光照射导致了水相中扁柏酚的分解。在光化学反应过程中，5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）与羟基自由基加合物的独特电子自旋共振信号伴随着与DMPO与超氧阴离子自由基加合物相对应的小信号。超过95%的大肠杆菌细胞在近紫外光照射下与扁柏酚一起孵育后被杀死。这些结果表明在近紫外光照射下扁柏酚的光化学反应过程中产生了活性氧物种。
