Cherepanova O A, Kalmykova N V, Blinova M I, Pinaev G P
Institute of Cytology RAS, St. Petersburg. cheer@mailru-com
Tsitologiia. 2003;45(3):271-6.
Laminins, as basal membrane glycoproteins, are able to stimulate cell adhesion and migration, and to influence gene expression. The laminin molecule has a set of bioactive sites that interact with different integrin and nonintegrin receptors, and, as a result, the reaction of the same cell type to different laminin isoforms may be different. The aim of this study was to determine the contributions of both integrins with beta 1 and beta 4 chains and 67 kDa laminin receptor in the interaction of A431 cells with two laminin isoforms: laminin-1 and laminin-2/4. The obtained data show that integrin alpha 6 beta 4 is more specific for interaction with laminin-2/4 than with laminin-1 and takes part in the stage of attachment of A431 cells to laminin. 67 kDa receptor promotes cell spreading on laminin-2/4 and inhibits cell spreading on laminin-1. An assumption was made about the complex action of receptors for interaction of A431 cells with laminins ("integrin alpha 6 beta 4--67 kDa receptors" complex).
层粘连蛋白作为基底膜糖蛋白，能够刺激细胞黏附和迁移，并影响基因表达。层粘连蛋白分子具有一组与不同整合素和非整合素受体相互作用的生物活性位点，因此，同一细胞类型对不同层粘连蛋白异构体的反应可能不同。本研究的目的是确定具有β1和β4链的整合素以及67 kDa层粘连蛋白受体在A431细胞与两种层粘连蛋白异构体（层粘连蛋白-1和层粘连蛋白-2/4）相互作用中的作用。获得的数据表明，整合素α6β4与层粘连蛋白-2/4相互作用比与层粘连蛋白-1更具特异性，并参与A431细胞与层粘连蛋白的附着阶段。67 kDa受体促进细胞在层粘连蛋白-2/4上的铺展，并抑制细胞在层粘连蛋白-1上的铺展。提出了关于A431细胞与层粘连蛋白相互作用的受体的复杂作用（“整合素α6β4-67 kDa受体”复合物）的假设。
