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Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae.

作者信息

Krizková L, Jeloková J, Klaudíny J, Zámocký M

机构信息

Laboratory of Genetic Engineering, Slovak Academy of Sciences, Bratislava.

出版信息

Folia Microbiol (Praha). 1992;37(4):273-8. doi: 10.1007/BF02814562.

DOI:10.1007/BF02814562
PMID:1452097
Abstract

A cDNA sequence coding for Japanese quail ovalbumin was used for the construction of expression plasmid under the ADH1 promoter of the yeast shuttle vector pVT101-U. The resulting recombinant expression vector pJK2 was used for the transformation of Saccharomyces cerevisiae. Expression of quail ovalbumin in yeast cells was demonstrated by Western blotting followed by immunochemical detection.

摘要

相似文献

1
Expression of Japanese quail ovalbumin in Saccharomyces cerevisiae.
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2
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引用本文的文献

1
Production and purification of Japanese quail ovalbumin as fusion protein with glutathione S-transferase in Escherichia coli.
Folia Microbiol (Praha). 1995;40(2):169-75. doi: 10.1007/BF02815417.

本文引用的文献

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The complete amino-acid sequence of hen ovalbumin.母鸡卵清蛋白的完整氨基酸序列。
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Synthesis of a chicken ovalbumin-like protein in the yeast Saccharomyces cerevisiae.在酿酒酵母中合成一种鸡卵清蛋白样蛋白。
Gene. 1980 Oct;11(1-2):163-7. doi: 10.1016/0378-1119(80)90096-7.
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Regulation of protein synthesis in chick oviduct. I. Independent regulation of ovalbumin, conalbumin, ovomucoid, and lysozyme induction.鸡输卵管中蛋白质合成的调控。I. 卵清蛋白、伴清蛋白、卵类黏蛋白和溶菌酶诱导的独立调控。
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