毛细管气相色谱法同时测定尿液中的2-甲氧基苯酚、2-甲氧基-4-甲基苯酚、2,6-二甲氧基苯酚和4'-羟基-3'-甲氧基苯乙酮

Simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4'-hydroxy-3'-methoxyacetophenone in urine by capillary gas chromatography.

作者信息

Bieniek Grazyna

机构信息

Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Oct 5;795(2):389-94. doi: 10.1016/s1570-0232(03)00593-2.

DOI:10.1016/s1570-0232(03)00593-2

PMID:14522045

Abstract

A method for the simultaneous determination of 2-methoxyphenol, 2-methoxy-4-methylphenol, 2,6-dimethoxyphenol and 4'-hydroxy-3'-methoxyacetophenone in urine has been described. The metabolites were analyzed after enzymatic hydrolysis and extraction on octyl (C8) cartridges by using gas chromatography with flame ionization detection and a 5/95% copolymer of diphenyl-poly(dimethylsiloxane) capillary column. Methoxyphenols were well separated within 12 min. Recovery was over 90% in the range from 0.5 to 20 microg/ml; the detection limit was varying in the range of 0.05-0.11 microg/ml. The relative standard deviations and the accuracy were in the range of 3.1-15.5 and 2.4-16.0%, respectively.

摘要

本文描述了一种同时测定尿液中2-甲氧基苯酚、2-甲氧基-4-甲基苯酚、2,6-二甲氧基苯酚和4'-羟基-3'-甲氧基苯乙酮的方法。代谢物经酶水解后，用火焰离子化检测气相色谱法和5/95%二苯基-聚（二甲基硅氧烷）共聚物毛细管柱在辛基（C8）柱上进行萃取分析。甲氧基苯酚在12分钟内得到良好分离。在0.5至20微克/毫升范围内回收率超过90%；检测限在0.05-0.11微克/毫升范围内变化。相对标准偏差和准确度分别在3.1-15.5%和2.4-16.0%范围内。