Compaan Bert, Ruttink Tom, Albrecht Cathy, Meeley Robert, Bisseling Ton, Franssen Henk
Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Biochim Biophys Acta. 2003 Oct 1;1629(1-3):84-91. doi: 10.1016/j.bbaexp.2003.08.004.
In Zea mays, two ENOD40 homologous were identified that show only 30% of sequence homology to each other. We identified line e40-mum1 carrying a Mu transposon inserted in ZmENOD40-1, the maize gene that has the highest homology to leguminous ENOD40. The insertion causes a dramatic reduction of the ZmENOD40-1 transcript level. Irrespective of this, homozygous e40-mum1 plants are still able to interact with mycorrhizal fungi. Furthermore, no phenotypic aberrations correlated to the presence of e40-mum1 have been identified and therefore it is suggested that Z. mays ENOD40 genes are functionally redundant despite their strikingly low homology.
在玉米中，鉴定出两个ENOD40同源基因，它们彼此之间的序列同源性仅为30%。我们鉴定出携带Mu转座子插入ZmENOD40-1的e40-mum1品系，ZmENOD40-1是与豆科植物ENOD40同源性最高的玉米基因。该插入导致ZmENOD40-1转录本水平急剧降低。尽管如此，纯合的e40-mum1植株仍能够与菌根真菌相互作用。此外，未发现与e40-mum1存在相关的表型异常，因此表明玉米ENOD40基因尽管同源性极低，但在功能上是冗余的。
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