Byrne M, Taylor W C
Cooperative Research Center for Plant Science, Australian National University, Canberra, Australia.
Mol Gen Genet. 1996 Aug 27;252(1-2):216-20. doi: 10.1007/s004389670025.
The recessive nuclear mutation iojap (ij) in maize produces striped plants with normal chloroplasts in green sectors and poorly developed chloroplasts in the white sectors. The ij mutation is also characterized by an array of additional phenotypic affects which suggests a pivotal role for Ij in chloroplast development. The Ij gene from maize has been isolated; however, the sequence has not provided information on the action of the Ij gene product. As an extension of the analysis of the Ij gene we have generated a number of independent transposon insertion mutations using the Robertson's Mutator (Mu). Insertions were found to be distributed throughout the 5' half of the gene and all showed a similar striping pattern to that of the original ij mutation, ij-ref. While several of the new mutant alleles produced ij-homologous transcripts none produced detectable levels of the Ij polypeptide product. Chloroplast development in some cells appears to be able to proceed in the absence of the Ij polypeptide.
玉米中的隐性核突变iojap（ij）产生的条纹植株，其绿色部分的叶绿体正常，而白色部分的叶绿体发育不良。ij突变还具有一系列其他表型效应，这表明Ij在叶绿体发育中起关键作用。玉米的Ij基因已被分离出来；然而，该序列并未提供有关Ij基因产物作用的信息。作为对Ij基因分析的扩展，我们利用罗伯逊氏转座子（Mu）产生了一些独立的转座子插入突变。发现插入分布在该基因5'端的一半区域，并且所有突变都表现出与原始ij突变（ij-ref）相似的条纹模式。虽然几个新的突变等位基因产生了与ij同源的转录本，但没有一个能产生可检测水平的Ij多肽产物。在某些细胞中，叶绿体发育似乎能够在没有Ij多肽的情况下进行。
