Libre Peter E, Della-Latta Phyllis, Chin Nai Xun
Columbia University, New York, New York, USA.
J Cataract Refract Surg. 2003 Sep;29(9):1791-4. doi: 10.1016/s0886-3350(03)00134-2.
To assess in vitro whether intracameral antibiotic agents are plausibly effective prophylaxis against Staphylococcus aureus endophthalmitis.
University research laboratory.Staphylococcus aureus was cultured for 8 hours with vancomycin (50 microg/mL) or piperacillin/tazobactam (512/64 microg/mL). Without reducing the bacterial counts, the antibiotic concentration was halved every 2 hours to simulate the half-life of antibiotic agents in the anterior chamber. The number of colony forming units (CFU)/mL was measured at 0, 2, 4, 6, and 8 hours.
Vancomycin inhibited the growth of methicillin-resistant S aureus such that the CFU/mL was 1000 times higher in the control group after 8 hours. Piperacillin/tazobactam nearly eliminated 1 oxacillin-susceptible strain and reduced the CFU/mL of another strain 1000-fold.
Intracameral vancomycin may inhibit the growth of S aureus in the anterior chamber after surgery. Inhibiting the growth could increase the chances that the eye's innate defenses would eliminate the bacterial contamination that could cause endophthalmitis.
在体外评估前房内抗生素制剂对金黄色葡萄球菌性眼内炎是否有合理有效的预防作用。
大学研究实验室。将金黄色葡萄球菌与万古霉素（50微克/毫升）或哌拉西林/他唑巴坦（512/64微克/毫升）培养8小时。在不降低细菌数量的情况下，每2小时将抗生素浓度减半，以模拟抗生素在前房中的半衰期。在0、2、4、6和8小时测量菌落形成单位（CFU）/毫升的数量。
万古霉素抑制耐甲氧西林金黄色葡萄球菌的生长，使得8小时后对照组的CFU/毫升高出1000倍。哌拉西林/他唑巴坦几乎消除了1株对苯唑西林敏感的菌株，并使另一菌株的CFU/毫升降低了1000倍。
前房内注射万古霉素可能会抑制术后前房内金黄色葡萄球菌的生长。抑制生长可能会增加眼部先天防御机制消除可能导致眼内炎的细菌污染的机会。
