实验性沙眼衣原体眼部感染期间抗原特异性B细胞的频率
Frequency of antigen-specific B cells during experimental ocular Chlamydia trachomatis infection.
作者信息
Pal S, Taylor H R, Huneke R B, Prendergast R A, Whittum-Hudson J A
机构信息
Immunology Laboratories, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
出版信息
Infect Immun. 1992 Dec;60(12):5294-7. doi: 10.1128/iai.60.12.5294-5297.1992.
Abstract
Chlamydia-specific antibody-secreting cells have been identified in conjunctiva and draining cervical lymph nodes by an ELISPOT assay in a cynomolgus monkey model of trachoma. These local sites contained numbers of chlamydia-specific B cells that were higher than those in distant inguinal lymph nodes and peripheral blood. The numbers of chlamydia-specific immunoglobulin G-secreting B cells observed were 5 to 57 per 10(6) cells in conjunctiva and 24 to 996 per 10(6) cells in cervical lymph nodes during conjunctival infection or after challenge of immune monkeys with the chlamydial 57-kDa heat shock protein (hsp60). These studies demonstrate a large chlamydia-specific B-cell component in the conjunctiva during ocular chlamydial infection. These results are similar to our findings for chlamydia-specific T-cell responses.
摘要
在沙眼的食蟹猴模型中，通过酶联免疫斑点试验（ELISPOT）在结膜和引流宫颈淋巴结中鉴定出沙眼衣原体特异性抗体分泌细胞。这些局部部位含有的沙眼衣原体特异性B细胞数量高于远处腹股沟淋巴结和外周血中的数量。在结膜感染期间或用衣原体57-kDa热休克蛋白（hsp60）攻击免疫猴后，在结膜中每10⁶个细胞中观察到的沙眼衣原体特异性分泌免疫球蛋白G的B细胞数量为5至57个，在宫颈淋巴结中为每10⁶个细胞24至996个。这些研究表明，眼部衣原体感染期间结膜中存在大量沙眼衣原体特异性B细胞成分。这些结果与我们关于沙眼衣原体特异性T细胞反应的发现相似。
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