Sanakoeva L P
Probl Tuberk Bolezn Legk. 2003(8):40-3.
In 185 children vaccinated with BCG, the phagocytic activity of leukocytes (PAL) was studied in different postimmunization periods. The modified procedure for examining PAL that reveals and assays specific phagocytic changes irrespective of the level of PAL was used. A suspended matter mixture of BCG mycobacteria (a specific object) and white saprophytic staphylococci (a non-specific object) was used as the objects of phagocytosis. A specific increase in PAL in the first 1.5 years after vaccination and the specific changes of phagocytosis during a vaccination process were first established. There was a close relationship of the specific changes of phagocytosis to the size of an infiltrate at the site of vaccination injection. The strain of antituberculosis immunity may be more objectively assessed by the phagocytic test than by the size of a skin scar and by the Mantoux test with 2TE PPD-L. The phagocytic test is recommended for individual monitoring of a antituberculosis vaccination process in children.
在185名接种卡介苗的儿童中，研究了不同免疫后时期白细胞的吞噬活性（PAL）。采用了改良的PAL检测程序，该程序可揭示并测定特定的吞噬变化，而不考虑PAL的水平。使用卡介苗分枝杆菌（特定对象）和白色腐生葡萄球菌（非特定对象）的悬浮物混合物作为吞噬对象。首次确定了接种疫苗后前1.5年内PAL的特异性增加以及接种过程中吞噬作用的特异性变化。吞噬作用的特异性变化与接种注射部位浸润的大小密切相关。与通过皮肤瘢痕大小和2TE PPD-L结核菌素试验相比，通过吞噬试验可以更客观地评估抗结核免疫菌株。建议采用吞噬试验对儿童抗结核疫苗接种过程进行个体监测。
