Baruah Gautam Lal, Belfort Georges
Howard P. Isermann Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Biotechnol Prog. 2003 Sep-Oct;19(5):1524-32. doi: 10.1021/bp030009f.
A methodology, called the aggregate transport model, is presented that can a priori predict both the pressure-independent permeation flux and yield of target species for the microfiltration of poly-disperse solutions. The model captures the phenomenon of critical shear rate. Beyond the critical shear rate (expressed as a ratio of shear rate to permeation flux), the transmission of proteins drops sharply as a result of cake classification. The widely reported benefits of operating at uniform transmembrane pressure and constant wall concentration follow from this method. The methodology is general in nature and can be used predictively to obtain an optimal balance between flux and yield of target species during the microfiltration of many commercial poly-disperse suspensions. In the accompanying paper we test this model for microfiltration of transgenic whole goat milk.
本文提出了一种称为总传输模型的方法，该方法可以先验地预测多分散溶液微滤过程中目标物质的压力无关渗透通量和产率。该模型捕捉了临界剪切速率现象。超过临界剪切速率（表示为剪切速率与渗透通量之比）时，由于滤饼分级，蛋白质的透过率会急剧下降。在均匀跨膜压力和恒定壁面浓度下操作所带来的诸多益处源于此方法。该方法本质上具有通用性，可用于预测性地在许多商业多分散悬浮液的微滤过程中获得目标物质通量和产率之间的最佳平衡。在随附的论文中，我们对该模型用于转基因全脂山羊奶的微滤进行了测试。
