Kim Dongjun, Kim Jeong Do, Baek Kwanghee, Yoon Yeup, Yoon Jaeseung
Graduate School of Biotechnology, Kyung Hee University, Yongin City, Kyungki-Do 449-701, Korea.
Biotechnol Prog. 2003 Sep-Oct;19(5):1620-2. doi: 10.1021/bp0341186.
Here we show that the transcriptional terminator element of human gastrin gene, which is the only element characterized to date in terms of its function in transcriptional termination, increases the transient expression levels of recombinant proteins. The expression of the beta-galactosidase gene was enhanced 3-4-fold in HeLa cells by inserting the terminator element of human gastrin gene at the 3'-side of the SV40 polyadenylation signal/cleavage site of the control vector (pSV-beta-gal). This effect of the terminator element is orientation-dependent but not cell-specific since a similar enhancement of beta-galactosidase gene expression was detected in COS.M6 and CHO DG44 cells. The increased level of beta-galactosidase gene expression by the transcriptional terminator element of human gastrin gene turned out to arise from elevated cellular mRNA levels, suggesting that the terminator element stabilizes mRNA by enhancing proper 3'-end processing of mRNA.
我们在此表明，人胃泌素基因的转录终止子元件可提高重组蛋白的瞬时表达水平，该元件是迄今为止在转录终止功能方面唯一被表征的元件。通过将人胃泌素基因的终止子元件插入对照载体（pSV-β-gal）的SV40多聚腺苷酸化信号/切割位点的3'侧，HeLa细胞中β-半乳糖苷酶基因的表达增强了3至4倍。终止子元件的这种作用是方向依赖性的，但不是细胞特异性的，因为在COS.M6和CHO DG44细胞中也检测到了β-半乳糖苷酶基因表达的类似增强。人胃泌素基因的转录终止子元件导致β-半乳糖苷酶基因表达水平升高，结果表明该终止子元件通过增强mRNA的正确3'末端加工来稳定mRNA。
