Pruñonosa J, Obach R, Diez-Cascón A, Gouesclou L
Pharmacokinetic Department, S.A. Lasa Laboratorios, Barcelona, Spain.
J Chromatogr. 1992 Oct 23;581(2):219-26. doi: 10.1016/0378-4347(92)80275-u.
A sensitive and selective high-performance capillary electrophoresis (HPCE) procedure was developed for the determination of total cicletanine in human plasma. The procedure consisted in extraction of the drug with diethyl ether and analysis by micellar electrokinetic capillary chromatography in a fused-silica capillary using sodium dodecyl sulphate in the run buffers and ultraviolet detection. The concentrations of cicletanine obtained by this method were compared with those obtained by a high-performance liquid chromatographic (HPLC) method used routinely. The within-run precision of the methods, expressed as relative standard deviation, ranged from 1.6 to 7.8% for HPLC and from 6.4 to 11.1% for HPCE. Both methods showed an adequate level of accuracy; the relative errors ranged from 0.02 to 3.25% for HPLC and from 0.21 to 2.90% for HPCE. The HPCE method required less than half the time taken by the HPLC method, making HPCE a useful alternative technique for the routine determination of cicletanine in plasma. Both methods were used to follow the time course of total cicletanine in human plasma after a single oral therapeutic dose of the drug.
建立了一种灵敏且选择性高的高效毛细管电泳（HPCE）方法，用于测定人血浆中的西氯他宁总量。该方法包括用乙醚提取药物，并在熔融石英毛细管中通过胶束电动毛细管色谱法进行分析，运行缓冲液中使用十二烷基硫酸钠，并采用紫外检测。将该方法测得的西氯他宁浓度与常规使用的高效液相色谱（HPLC）方法测得的浓度进行比较。以相对标准偏差表示，两种方法的批内精密度范围为：HPLC为1.6%至7.8%，HPCE为6.4%至11.1%。两种方法均显示出足够的准确度；相对误差范围为：HPLC为0.02%至3.25%，HPCE为0.21%至2.90%。HPCE方法所需时间不到HPLC方法的一半，这使得HPCE成为血浆中西氯他宁常规测定的一种有用替代技术。两种方法均用于跟踪单次口服治疗剂量该药物后人血浆中西氯他宁总量的时间进程。
