Sutton B M, Richardson R A
Pharmaceuticals and Toxicology Section, DSIR Chemistry, Petone, New Zealand.
J Chromatogr. 1992 Oct 23;581(2):277-80. doi: 10.1016/0378-4347(92)80282-u.
A simple and sensitive gas chromatographic method has been developed for the determination of timolol in plasma using electron-capture detection and propranolol as internal standard. Timolol was extracted using butyl chloride and derivatized using trifluoroacetic anhydride in butyl acetate. The lower detection limit for the assay was found to be 1 ng/ml from 1 ml of plasma. Extracted standards gave within-day precision of 12.55, 9.68 and 3.78% for 1, 20 and 100 ng/ml plasma samples, respectively. A recovery of at least 80% of timolol was found using the extraction method described. The assay was used in a randomized cross-over bioequivalence trial using an oral administration of 20 mg of timolol. Pharmacokinetic parameters compare favourably with other literature values.
已开发出一种简单且灵敏的气相色谱法，用于测定血浆中的噻吗洛尔，采用电子捕获检测法，并以普萘洛尔作为内标。噻吗洛尔用氯丁烷萃取，并用乙酸丁酯中的三氟乙酸酐进行衍生化。该测定法的最低检测限为1毫升血浆中1纳克/毫升。对于1、20和100纳克/毫升的血浆样品，萃取标准品的日内精密度分别为12.55%、9.68%和3.78%。使用所述萃取方法发现噻吗洛尔的回收率至少为80%。该测定法用于一项口服20毫克噻吗洛尔的随机交叉生物等效性试验。药代动力学参数与其他文献值相比具有优势。
