酶联免疫吸附测定法检测传染性软疣病毒IgG抗体及分子型血清学关系研究

Enzyme-linked immunosorbent assay for measurement of IgG antibody to Molluscum contagiosum virus and investigation of the serological relationship of the molecular types.

作者信息

Konya J, Thompson C H, De Zwart-Steffe R T

机构信息

Department of Infectious Diseases, University of Sydney, New South Wales, Australia.

出版信息

J Virol Methods. 1992 Nov;40(2):183-94. doi: 10.1016/0166-0934(92)90067-n.

DOI:10.1016/0166-0934(92)90067-n

PMID:1452633

Abstract

An ELISA was developed for measuring levels of IgG antibodies to Molluscum contagiosum virus (MCV) using DNA typed purified virus as antigen. The assay was found to be specific and sensitive for antibodies to MCV as well as being economical in its use of antigen. A close relationship was found between antibody levels to the MCV molecular types 1, 1v and 2 by cross-testing sera on plates coated with the different molecular types of the virus as antigen (P < 0.001).

摘要

开发了一种酶联免疫吸附测定法（ELISA），以DNA分型的纯化病毒为抗原，用于检测抗传染性软疣病毒（MCV）的IgG抗体水平。该测定法被发现对MCV抗体具有特异性和敏感性，并且在抗原使用方面较为经济。通过在包被有不同分子类型病毒作为抗原的平板上交叉检测血清，发现针对MCV分子类型1、1v和2的抗体水平之间存在密切关系（P < 0.001）。