用酶法合成CCK-8四肽片段。

Synthesis of CCK-8 tetrapeptide fragment by enzymatic method.

作者信息

Xiang Guangya, Eckstein Heiner

机构信息

Pharmacy School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):234-5, 241. doi: 10.1007/BF02829500.

DOI:10.1007/BF02829500

PMID:14526420

Abstract

The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.

摘要

报道了胆囊收缩素C端八肽CCK-8片段四肽Phac-Met-Gly-Trp-Met-OEt的酶促合成。该片段通过依次将Phac-Met-OEt与Gly-OMe、Trp-OMe和Met-OEt偶联来合成。这三个步骤分别由α-胰凝乳蛋白酶、木瓜蛋白酶和α-胰凝乳蛋白酶催化。快原子轰击质谱（FAB-MS）结果表明，所有产物均具有正确的分子量。