Liu Xiaocheng, Yang Cheng, He Xiaofeng
Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
J Huazhong Univ Sci Technolog Med Sci. 2003;23(3):271-4. doi: 10.1007/BF02829511.
The expression, activity and clinical implication of heme oxygenase-1 (HO-1) in the chronic renal insufficiency (CRI) rat kidney and its mechanism were investigated. The 5/6 nephrectomized rats were assigned to sham operation group, CRI group and Hemin group. At the 8th week after second operation, blood pressure, urinary protein, serum creatinine (Scr) and BUN were measured. Renal pathologic changes were observed. The activity of HO and contents of erythropoietin (EPO) in serum and renal tissue were determined. Immunohistochemistry was used to detect the expression and distribution of HO-1 in the CRI rat kidney. As compared with CRI group, the urinary protein, blood pressure, Scr and BUN in Hemin group were reduced significantly (P < 0.05). The glomerular mesangial proliferation, inflammatory cellular infiltration of renal interstitium and interstitial fibrosis were ameliorated significantly. Immunohistochemistry and measurement of HO-1 activity revealed that the expression and activity of HO-1 was decreased in renal tissues and increased in serum in CRI group as compared with normal rats. HO-1 distributed mainly in tubular epithelial cells. The EPO contents in Hemin group were significantly higher than in CRI group. Through up-regulating the EPO level in serum and renal tissues, HO-1 retards the progression of CRI.
研究血红素加氧酶-1（HO-1）在慢性肾功能不全（CRI）大鼠肾脏中的表达、活性及临床意义及其机制。将5/6肾切除大鼠分为假手术组、CRI组和血红素组。二次手术后第8周，测量血压、尿蛋白、血清肌酐（Scr）和尿素氮（BUN）。观察肾脏病理变化。测定血清和肾组织中HO的活性及促红细胞生成素（EPO）的含量。采用免疫组织化学法检测HO-1在CRI大鼠肾脏中的表达和分布。与CRI组相比，血红素组的尿蛋白、血压、Scr和BUN显著降低（P<0.05）。肾小球系膜增生、肾间质炎性细胞浸润和间质纤维化明显改善。免疫组织化学和HO-1活性测定显示，与正常大鼠相比，CRI组肾组织中HO-1的表达和活性降低，血清中HO-1的表达和活性升高。HO-1主要分布在肾小管上皮细胞。血红素组的EPO含量明显高于CRI组。HO-1通过上调血清和肾组织中的EPO水平，延缓CRI的进展。
