[酵母中木糖还原酶和木糖醇脱氢酶的活性]
[The activity of xylose reductase and xylitol dehydrogenase in yeasts].
作者信息
Iablochkova E N, Bolotnikova O I, Mikhaĭlova N P, Nemova N N, Ginak A I
机构信息
St. Petersburg State Technological Institute (Technical University), Moskovskii pr. 26, St. Petersburg, 198013 Russia.
出版信息
Mikrobiologiia. 2003 Jul-Aug;72(4):466-9.
The activity and the cofactor specificity of xylose reductase and xylitol dehydrogenase were studied in extracts of yeasts from the genera Candida, Kluyveromyces, Pachysolen, Pichia, and Torulopsis grown under microaerobic conditions. It was found that xylitol dehydrogenase in all of the yeast species studied is specific for NAD+; xylose reductase in the xylitol-producing species C. didensiae, C. intermediae, C. parapsilosis, C. silvanorum, C. tropicalis, Kl. fragilis, Kl. marxianus, P. guillermondii, and T. molishiama is specific for NADPH; and xylose reductase in the ethanol-producing species P. stipitis, C. shehatae, and Pa. tannophilus is specific for both NADPH and NADH.
在微需氧条件下培养的念珠菌属、克鲁维酵母属、毕赤酵母属、毕赤酵母属和球拟酵母属酵母提取物中，研究了木糖还原酶和木糖醇脱氢酶的活性及辅因子特异性。结果发现，在所研究的所有酵母物种中，木糖醇脱氢酶对NAD⁺具有特异性；在产木糖醇的物种迪登斯念珠菌、中间念珠菌、近平滑念珠菌、西尔瓦诺念珠菌、热带念珠菌、脆壁克鲁维酵母、马克斯克鲁维酵母、季也蒙毕赤酵母和莫氏球拟酵母中，木糖还原酶对NADPH具有特异性；在产乙醇的物种树干毕赤酵母、谢哈塔念珠菌和嗜单宁毕赤酵母中，木糖还原酶对NADPH和NADH均具有特异性。
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