Il'chenko A P, Cherniavskaia O G, Shishkanova N V, Finogenova T V
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
Mikrobiologiia. 2003 Jul-Aug;72(4):470-5.
A comparative assay of nitrogen metabolism enzymes in the Yarrowia lipolytica mutant N1 grown under conditions promoting the overproduction of either alpha-ketoglutaric acid (KGA) or citric acid showed that the overproduction of KGA correlates with an increase in the activities of the NAD- and NADP-linked glutamate dehydrogenase, glutamic-pyruvic transaminase, and glutamic-oxaloacetic transaminase reactions. These reactions are likely to be responsible for the overproduction of KGA by this mutant. In contrast, the overproduction of citric acid correlated with a decline in the activities of the NAD- and NADP-linked glutamate dehydrogenases and with an increase in the activities of glutamine synthetase and glutamate synthase.
对在促进α-酮戊二酸（KGA）或柠檬酸过量产生的条件下生长的解脂耶氏酵母突变体N1中的氮代谢酶进行的比较测定表明，KGA的过量产生与NAD和NADP连接的谷氨酸脱氢酶、谷丙转氨酶和谷草转氨酶反应活性的增加相关。这些反应可能是该突变体过量产生KGA的原因。相比之下，柠檬酸的过量产生与NAD和NADP连接的谷氨酸脱氢酶活性的下降以及谷氨酰胺合成酶和谷氨酸合酶活性的增加相关。
