利用鼠伤寒沙门氏菌TA1535/pSK1002 umu试验研究梅（Prunus mume Sieb. Et Zucc.）中柠檬酸酯对化学诱变剂SOS诱导活性的抑制作用。
Suppressive effect of the SOS-inducing activity of chemical mutagen by citric acid esters from Prunus mume Sieb. Et Zucc. using the Salmonella typhimurium TA1535/pSK1002 umu test.
作者信息
Miyazawa Mitsuo, Yamada Tomoki, Utsunomiya Hirotoshi
机构信息
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka-shi, Osaka 577-8502, Japan.
出版信息
Nat Prod Res. 2003 Oct;17(5):319-23. doi: 10.1080/1057563031000072550.
A methanol extract from Prunus mume Sieb. Et Zucc. showed a suppressive effect of the SOS-inducing activity on the mutagen 3-amino-1,4-dimethyl-SH-pyrido[4,3-b]indole(Trp-P-1) in the Salmonella typhimurium TA1535/pSK1002 umu test. The methanol extract was re-extracted with hexane, dichloromethane, ethyl acetate, butanol and water. The dichloromethane and ethyl acetate fractions showed suppressive effect. Suppressive compounds were isolated by silica gel column chromatography and identified as trimethyl citrate (1) and dimethyl citrate (2) by GC-MS, IR and 1H and 13C-NMR spectroscopy. Compounds 1 and 2 suppressed 51 and 39% of the SOS-inducing activity at a concentration of 2.0 micromol/mL.
梅（Prunus mume Sieb. Et Zucc.）的甲醇提取物在鼠伤寒沙门氏菌TA1535/pSK1002 umu试验中，对诱变剂3-氨基-1,4-二甲基-SH-吡啶并[4,3-b]吲哚（Trp-P-1）的SOS诱导活性具有抑制作用。将该甲醇提取物依次用己烷、二氯甲烷、乙酸乙酯、丁醇和水进行再萃取。二氯甲烷和乙酸乙酯部分显示出抑制作用。通过硅胶柱色谱法分离出具有抑制作用的化合物，并通过气相色谱-质谱联用（GC-MS）、红外光谱（IR）、氢核磁共振（1H-NMR）和碳核磁共振（13C-NMR）光谱鉴定为柠檬酸三甲酯（1）和柠檬酸二甲酯（2）。化合物1和2在浓度为2.0微摩尔/毫升时，分别抑制了51%和39%的SOS诱导活性。
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