Girard C, Miane J M, Riou J, Baret R
C R Seances Soc Biol Fil. 1977;171(4):836-46.
By cellulose acetate electrophoresis we have confirmed the presence of two desamidinasic fractions in aqueous extracts of acetone powder from pig liver. Pig kidney extracts have been partially purified by thermal denaturation and chromatography on Sephadex G-200 and D.E.A.E. Sephadex A-50. Michaelis constants of the two eluted arginasic fractions have been determined.
通过醋酸纤维素电泳,我们已证实在猪肝丙酮粉的水提取物中存在两种脱脒基酶组分。猪肾提取物已通过热变性以及在葡聚糖凝胶G - 200和二乙氨基乙基葡聚糖凝胶A - 50上的色谱法进行了部分纯化。已测定了两种洗脱的精氨酸酶组分的米氏常数。