来自阔节裂头绦虫的28.0S核糖体RNA中的一个隐蔽断裂点。

A hidden break in the 28.0S rRNA from Diphyllobothrium dendriticum.

作者信息

Karlstedt K A, Paatero G I, Mäkelä J H, Wikgren B J

机构信息

Department of Biology, Abo Akademi University, Finland.

出版信息

J Helminthol. 1992 Sep;66(3):193-7. doi: 10.1017/s0022149x00014553.

DOI:10.1017/s0022149x00014553

PMID:1452994

Abstract

Nondenatured and denatured total RNA from the tapeworm Diphyllobothrium dendriticum (Cestoda) was analysed by agarose gel electrophoresis. It was found that the large subunit ribosomal RNA (lrRNA) is 28.0S and the small subunit ribosomal RNA (srRNA) is 19.5S. Following denaturation the 28.0S rRNA was disrupted into a 19.5S subfragment and a 20.7S subfragment due to the presence of a centrally located hidden break. By hybridization of Northern blot membranes with oligonucleotide probes specific for the 5'- and 3'-ends of the lrRNA respectively, we have shown that the 19.5S subfragment is from the 5'-end (the alpha-subfragment) and the 20.7S subfragment from the 3'-end (the beta-subfragment) of the 28.0S rRNA of D. dendriticum.

摘要

通过琼脂糖凝胶电泳分析了树枝状双槽绦虫（绦虫纲）的未变性和变性总RNA。结果发现，核糖体大亚基RNA（lrRNA）为28.0S，核糖体小亚基RNA（srRNA）为19.5S。变性后，由于存在位于中央的隐藏断裂，28.0S rRNA被裂解为一个19.5S亚片段和一个20.7S亚片段。通过分别用对lrRNA的5'端和3'端特异的寡核苷酸探针与Northern印迹膜杂交，我们已经表明19.5S亚片段来自树枝状双槽绦虫28.0S rRNA的5'端（α-亚片段），而20.7S亚片段来自3'端（β-亚片段）。