Tomaszewska Agnieszka, Guevara Ibeth, Wilczok Tadeusz, Dembińska-Kieć Aldona
Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland.
Gynecol Obstet Invest. 2003;56(3):152-9. doi: 10.1159/000073775. Epub 2003 Sep 29.
The present study investigated the effect of 17beta-estradiol (17betaE(2)) and lipopolysaccharide (LPS) on the release and expression of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and vascular endothelial growth factor (VEGF) in the macrophage cell line RAW 264.7. The cells were pre-incubated with 17betaE(2) at physiological as well as supraphysiological concentrations (12.5-1000 pg/ml) and subsequently activated with LPS (100 ng/ml). The changes in NO, TNF-alpha and VEGF release into culture medium and also their gene expression in cells were assessed. A concentration-dependent inhibitory effect of 17betaE(2) on NO and TNFalpha release was observed at low physiological concentrations, whereas the VEGF gene expression and protein levels were upregulated by 17betaE(2) in RAW 264.7 cells.
本研究调查了17β-雌二醇（17βE₂）和脂多糖（LPS）对巨噬细胞系RAW 264.7中一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和血管内皮生长因子（VEGF）释放及表达的影响。将细胞分别用生理浓度及超生理浓度（12.5 - 1000 pg/ml）的17βE₂预孵育，随后用LPS（100 ng/ml）激活。评估了培养基中NO、TNF-α和VEGF释放的变化以及它们在细胞中的基因表达。在低生理浓度下观察到17βE₂对NO和TNF-α释放具有浓度依赖性抑制作用，而在RAW 264.7细胞中，17βE₂上调了VEGF基因表达和蛋白水平。
