卵母细胞预培养时间对体外受精和卵胞浆内单精子注射中核成熟度、受精率、胚胎质量及妊娠结局的影响。
The effect of preincubation period of oocytes on nuclear maturity, fertilization rate, embryo quality, and pregnancy outcome in IVF and ICSI.
作者信息
Ho Jason Yen-Ping, Chen Ming-Jer, Yi Yu-Chiao, Guu Hwa-Fen, Ho Esther Shih-Chu
机构信息
Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, 160, Sec. 3, Chung-Kang Road, Taichung 407, Taiwan, Republic of China.
出版信息
J Assist Reprod Genet. 2003 Sep;20(9):358-64. doi: 10.1023/a:1025476910771.
PURPOSE
To clarify the effect of preincubation of oocytes on the results of IVF and ICSI.
METHODS
A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm; fertilization rates, embryo quality, and pregnancy outcomes were analyzed in both IVF and ICSI arms according to the preincubation period duration of oocytes.
RESULTS
The MII percentage of the ICSI arm was significantly lower (P < 0.05) in the group with preincubation period of < 2.5 h. The fertilization rates in groups with preincubation for 2.5-5.5 h were significantly higher (P < 0.001) for IVF. Embryo quality and pregnancy outcomes were not significantly different between the IVF or ICSI arm.
CONCLUSIONS
The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.
目的
阐明卵母细胞预孵育对体外受精（IVF）和卵胞浆内单精子注射（ICSI）结果的影响。
方法
共有176个IVF周期和64个ICSI周期接受了长方案卵巢刺激。卵母细胞在授精或精子注射前孵育1 - 8小时。在ICSI组中评估中期II（MII）百分比；根据卵母细胞的预孵育时间，在IVF和ICSI组中分析受精率、胚胎质量和妊娠结局。
结果
在预孵育时间<2.5小时的组中，ICSI组的MII百分比显著降低（P < 0.05）。对于IVF，预孵育2.5 - 5.5小时组的受精率显著更高（P < 0.001）。IVF组或ICSI组之间的胚胎质量和妊娠结局无显著差异。
结论
卵母细胞预孵育至少2.5小时通过提高卵母细胞核成熟度对IVF和ICSI结局均有益。
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