卵丘细胞对体外细胞质成熟和卵母细胞发育能力的贡献。
Cumulus cell contribution to cytoplasmic maturation and oocyte developmental competence in vitro.
作者信息
Hassan H A
机构信息
Obstetrics & Gynecology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
出版信息
J Assist Reprod Genet. 2001 Oct;18(10):539-43. doi: 10.1023/a:1011901923261.
Abstract
OBJECTIVE
Although several reports indicated the bidirectional communication between the oocyte and granulosa cell, and the potential negative impact of premature cumulus removal, clinical data are controversial. The present analysis report the results of a prospective randomized study done on sibling oocytes to evaluate whether or not preincubation with intact cumulus is needed in ICSI.
METHODS
It was a prospective randomized study, and conducted at Alexandria IVF-ICSI center. This study was performed using 926 M2 oocytes from 141 cycles of patients undergoing ICSI. Oocytes were randomized between three protocols: protocol A (immediate denudation, immediate injection), protocol B (delayed denudation, delayed injection), and protocol C (early denudation, delayed injection). Main outcome measure(s) were maturation, fertilization, and cleavage rate after ICSI.
RESULTS
Aspects of nuclear, cytoplasmic maturation and oolemma properties were improved when oocytes were preincubated with intact cumulus before ICSI.
CONCLUSIONS
Preincubation, before ICSI improved results. Duration of preincubation should be program specific.
摘要
目的
尽管有几份报告指出卵母细胞与颗粒细胞之间存在双向通讯，以及过早去除卵丘的潜在负面影响，但临床数据仍存在争议。本分析报告了一项针对同胞卵母细胞进行的前瞻性随机研究结果，以评估在卵胞浆内单精子注射（ICSI）中是否需要与完整卵丘进行预孵育。
方法
这是一项前瞻性随机研究，在亚历山大IVF-ICSI中心进行。本研究使用了来自141例接受ICSI治疗患者周期的926个M2期卵母细胞。卵母细胞被随机分为三种方案：方案A（立即去卵丘、立即注射）、方案B（延迟去卵丘、延迟注射）和方案C（早期去卵丘、延迟注射）。主要观察指标为ICSI后的成熟率、受精率和卵裂率。
结果
在ICSI前将卵母细胞与完整卵丘进行预孵育时，核、细胞质成熟及卵膜特性等方面均得到改善。
结论
ICSI前的预孵育可改善结果。预孵育时间应因方案而异。
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