糖尿病大鼠双糖酶活性的昼夜变化及升高

Diurnal variation and increase of disaccharidase activity in diabetic rats.

作者信息

Nashiro K, Murakami K, Mimura G

机构信息

Second Department of Internal Medicine, School of Medicine, University of the Ryukyus, Okinawa, Japan.

出版信息

J Nutr Sci Vitaminol (Tokyo). 1992 Jun;38(3):265-76. doi: 10.3177/jnsv.38.265.

DOI:10.3177/jnsv.38.265

PMID:1453237

Abstract

The small intestinal disaccharidase activity and its daily variation in the diabetic rat have not been well described. Therefore, the small intestinal disaccharidase (maltase, lactase and sucrase) activity and its daily profile were studied in streptozotocin-induced diabetic rats under physiological conditions. In diabetic rats, a similar pattern of diurnal variation of disaccharidase activity to control rats was observed, while the relationships between daily change of disaccharidase activity and that of food consumption suggested that there was a different mechanism of diurnal variation in diabetic rats. On the other hand, a significant increase of mean 24-h lactase and sucrase activities was noted in diabetic rats, while that of maltase was not significant. Using the in vitro incubation method, a significant correlation between glucose concentration and lactase or sucrase activity but not maltase activity was observed. However, insulin showed no effect on disaccharidase activity. Thus we clarified the presence of a diurnal variation of disaccharidase activity and an increase in its activity in diabetic rats. This change was suggested to be derived from high plasma glucose level.

摘要

糖尿病大鼠小肠双糖酶活性及其每日变化情况尚未得到充分描述。因此，我们研究了链脲佐菌素诱导的糖尿病大鼠在生理条件下小肠双糖酶（麦芽糖酶、乳糖酶和蔗糖酶）活性及其每日变化情况。在糖尿病大鼠中，观察到双糖酶活性的昼夜变化模式与对照大鼠相似，而双糖酶活性的每日变化与食物摄入量之间的关系表明，糖尿病大鼠昼夜变化的机制有所不同。另一方面，糖尿病大鼠的平均24小时乳糖酶和蔗糖酶活性显著增加，而麦芽糖酶活性则无显著变化。采用体外孵育法，观察到葡萄糖浓度与乳糖酶或蔗糖酶活性之间存在显著相关性，但与麦芽糖酶活性无关。然而，胰岛素对双糖酶活性没有影响。因此，我们明确了糖尿病大鼠双糖酶活性存在昼夜变化且其活性增加。这种变化被认为是由高血糖水平引起的。