Kumar Shetty Ajaya, Suresh Kumar Gurusiddaiah, Veerayya Salimath Paramahans
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
Mol Nutr Food Res. 2005 Aug;49(8):791-6. doi: 10.1002/mnfr.200500035.
During diabetes, structural and functional changes in the alimentary tract are known to take place resulting in increased absorption of intestinal glucose and alterations in the activities of brush border disaccharidases. Similar observations are also reported in the renal cortex. In the present investigation, we examined the effect of feeding bitter gourd fruit devoid of seeds on activities of intestinal and renal disaccharidases, viz., maltase, sucrase, and lactase in streptozotocin-induced diabetic rats. Normal and diabetic rats were fed either with basal diet or a diet containing 10% bitter gourd powder. Specific activities of intestinal disaccharidases were significantly increased during diabetes, and supplementing bitter gourd in the diet clearly indicated amelioration in the activities of maltase and lactase during diabetes. However, a significant change was not observed with sucrase activity by feeding of bitter gourd. During diabetes, renal disaccharidase activities were significantly lower than those in the control rats. Bitter gourd supplementation was beneficial in alleviating the reduction in maltase activity during diabetes. However, not much change in the activities of sucrase and lactase was observed upon feeding. This positive influence of feeding bitter gourd on intestinal and renal disaccharidases clearly indicates their beneficial role in the management of diabetes, thus making diabetic animals more tolerant to hyperglycemia.
在糖尿病期间，已知消化道会发生结构和功能变化，导致肠道葡萄糖吸收增加以及刷状缘双糖酶活性改变。在肾皮质中也有类似的观察结果。在本研究中，我们研究了给链脲佐菌素诱导的糖尿病大鼠喂食无籽苦瓜果实对肠道和肾脏双糖酶（即麦芽糖酶、蔗糖酶和乳糖酶）活性的影响。正常大鼠和糖尿病大鼠分别喂食基础饮食或含10%苦瓜粉的饮食。糖尿病期间肠道双糖酶的比活性显著增加，饮食中添加苦瓜清楚地表明糖尿病期间麦芽糖酶和乳糖酶的活性有所改善。然而，喂食苦瓜对蔗糖酶活性未观察到显著变化。糖尿病期间，肾脏双糖酶活性显著低于对照大鼠。补充苦瓜有利于减轻糖尿病期间麦芽糖酶活性的降低。然而，喂食后蔗糖酶和乳糖酶的活性变化不大。喂食苦瓜对肠道和肾脏双糖酶的这种积极影响清楚地表明它们在糖尿病管理中的有益作用，从而使糖尿病动物对高血糖更具耐受性。
