Savouré N, Maudet M, Nicol M
Biochimie Médicale A, Faculté de Médecine de Rennes, France.
J Toxicol Clin Exp. 1992 May;12(2):97-108.
After a chloroform intraperitoneal injection, lactate dehydrogenase, alanine aminotransferase and particularly aspartate aminotransferase serum activities are much more raised in deficient animals. Liver ornithine decarboxylase (ODC) activity normally decreases in rats between the 4th. and the 7th. month after the weaning. In vitamin A deficient animals, basal values of the enzyme activity are lower and the decrease is deeper. But even at month 7, liver sustains a partial capacity of ODC recovery if retinol is fed during 15 days. Chloroform administration strongly enhances liver ODC activity in normal rats. In the deficiency, stimulation is lower in absolute value but relatively higher if referred to basal level. After retinol refeeding, chloroform stimulates enzyme activity to nearly normal values. Vitamin A deficiency impairs obviously liver ODC activity and its response to chloroform stimulation in rats, but the stroke is at least partially reversible in our conditions. Moreover, deficient animals maintain a non negligible capacity of ODC response under chloroform stimulation.
腹腔注射氯仿后，缺乏维生素A的动物血清中乳酸脱氢酶、丙氨酸转氨酶尤其是天冬氨酸转氨酶的活性会显著升高。正常情况下，大鼠断奶后第4至7个月肝脏鸟氨酸脱羧酶（ODC）活性会降低。在维生素A缺乏的动物中，该酶活性的基础值较低且下降幅度更大。但即使到了第7个月，如果在15天内喂食视黄醇，肝脏仍能部分恢复ODC活性。给正常大鼠注射氯仿会强烈增强肝脏ODC活性。在维生素A缺乏的情况下，刺激的绝对值较低，但相对于基础水平则较高。重新喂食视黄醇后，氯仿可将酶活性刺激至接近正常水平。维生素A缺乏明显损害大鼠肝脏ODC活性及其对氯仿刺激的反应，但在我们的实验条件下，这种损害至少部分是可逆的。此外，缺乏维生素A的动物在氯仿刺激下仍保持不可忽视的ODC反应能力。
