House A, Gregory S P, Catchpole B
Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA.
Vet Rec. 2003 Sep 20;153(12):354-8. doi: 10.1136/vr.153.12.354.
The pattern of expression of cytokine mRNA in the lesions of anal furunculosis was evaluated in tissue biopsies from 15 dogs, and compared with the pattern in control skin samples from 24 dogs, by reverse-transcriptase PCR using canine cytokine-specific primers and a semi-quantitative multiplex PCR assay. Interleukin-2 (IL-2) was detected in 11 of the 15 affected dogs but in only one of the controls, and interferon-gamma was detected in 14 of the affected dogs but none of the controls. In contrast, IL-4 was detected only in one of the affected dogs. Increased expression of mRNA for IL-1beta, IL-6, tumour necrosis factor alpha, IL-8, IL-10 and transforming growth factor beta1 was detected in the biopsies from the lesions of anal furunculosis relative to the control tissues (P < 0.05).
通过使用犬细胞因子特异性引物的逆转录聚合酶链反应和半定量多重聚合酶链反应分析，对15只患肛门疖病犬的组织活检样本中细胞因子mRNA的表达模式进行了评估，并与24只对照犬的皮肤样本中的模式进行了比较。在15只患病犬中的11只检测到白细胞介素-2（IL-2），但在对照犬中仅1只检测到；在14只患病犬中检测到干扰素-γ，而对照犬中均未检测到。相比之下，仅在1只患病犬中检测到IL-4。相对于对照组织，在肛门疖病病变的活检样本中检测到IL-1β、IL-6、肿瘤坏死因子α、IL-8、IL-10和转化生长因子β1的mRNA表达增加（P<0.05）。
