Hou Rolis Chien-Wei, Chen Huan-Lian, Tzen Jason T C, Jeng Kee-Ching G
Graduate Institute of Biotechnology, National Chung Hsing University, Taiwan, RO China.
Neuroreport. 2003 Oct 6;14(14):1815-9. doi: 10.1097/00001756-200310060-00011.
Sesame antioxidants have been shown to inhibit lipid peroxidation and regulate cytokine production. In this study, we focused on the effect of sesamin and sesamolin, on nitric oxide (NO) induction by lipopolysaccharides (LPS) in the murine microglial cell line BV-2 and rat primary microglia. The results showed that sesamin and sesamolin significantly inhibited NO production, iNOS mRNA and protein expression in LPS-stimulated BV-2 cells. Sesamin or sesamolin significantly reduced LPS-activated p38 MAPK of BV-2 cells. Furthermore, SB203580, a specific inhibitor of p38 MAP kinase, dose-dependently inhibited NO production in LPS-stimulated BV-2 cells. Taken together, the inhibition of NO production might be due to the reduction of LPS-induced p38 MAPK signal pathway by sesamin and sesamolin.
芝麻抗氧化剂已被证明可抑制脂质过氧化并调节细胞因子的产生。在本研究中，我们重点关注芝麻素和芝麻林素对小鼠小胶质细胞系BV-2和大鼠原代小胶质细胞中脂多糖（LPS）诱导的一氧化氮（NO）生成的影响。结果表明，芝麻素和芝麻林素显著抑制LPS刺激的BV-2细胞中NO的产生、iNOS mRNA和蛋白表达。芝麻素或芝麻林素显著降低LPS激活的BV-2细胞中的p38 MAPK。此外，p38 MAP激酶的特异性抑制剂SB203580剂量依赖性地抑制LPS刺激的BV-2细胞中NO的产生。综上所述，NO产生的抑制可能是由于芝麻素和芝麻林素降低了LPS诱导的p38 MAPK信号通路。
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