Natsugoe Shoji, Nakashima Saburo, Nakajo Akihiro, Matsumoto Masataka, Okumura Hiroshi, Tokuda Koki, Miyazono Futoshi, Kijima Fumio, Aridome Kuniaki, Ishigami Sumiya, Takao Sonshin, Aikou Takashi
First Department of Surgery, Kagoshima University School of Medicine, Kagoshima, Japan.
Oncol Rep. 2003 Nov-Dec;10(6):1879-83. doi: 10.3892/or.10.6.1879.
The clinical implications of bone marrow micrometastases (BMM) detected by RT-PCR in esophageal squamous cell carcinoma (ESCC) have not been elucidated. We evaluated the relation between the presence of BMM, both before and after surgery, and clinicopathologic findings in patients with ESCC. Bone marrow samples from 48 patients with ESCC were obtained from the iliac crest before and after surgery. After total RNA was extracted from each bone marrow sample, carcinoembryonic antigen (CEA)-specific RT-PCR was performed. BMM was detected by RT-PCR in 10 of the 48 patients. Four patients each had positive signals only before or only after surgery and 2 patients had positive signals both before and after surgery. There were no significant differences in clinicopathologic factors, including neoadjuvant therapy, between patients with BMM and without BMM. To date, the rates of recurrent disease in patients with BMM and without BMM are 80% (8/10) and 50% (19/38), respectively, a difference which is not significant. The 4-year survival rates of patients with BMM and without BMM are 10.0% and 47.3%, respectively. Recurrence and survival rates were poorer in patients with RT-PCR positivity, although the differences were not significant. A larger study is required to clarify the clinical impact of BMM.
通过逆转录聚合酶链反应（RT-PCR）检测到的食管鳞状细胞癌（ESCC）骨髓微转移（BMM）的临床意义尚未阐明。我们评估了ESCC患者手术前后BMM的存在与临床病理特征之间的关系。从48例ESCC患者的髂嵴获取手术前后的骨髓样本。从每个骨髓样本中提取总RNA后，进行癌胚抗原（CEA）特异性RT-PCR。48例患者中有10例通过RT-PCR检测到BMM。4例患者仅在手术前或仅在手术后有阳性信号，2例患者手术前后均有阳性信号。有BMM和无BMM的患者在包括新辅助治疗在内的临床病理因素方面无显著差异。迄今为止，有BMM和无BMM的患者复发率分别为80%（8/10）和50%（19/38），差异不显著。有BMM和无BMM的患者4年生存率分别为10.0%和47.3%。RT-PCR阳性患者的复发率和生存率较差，尽管差异不显著。需要进行更大规模的研究以阐明BMM的临床影响。
