Walters E, Buchheit K, Maruniak J A
Division of Biological Sciences, University of Missouri, Columbia 65211.
J Neurosci Res. 1992 Sep;33(1):103-11. doi: 10.1002/jnr.490330113.
Immunohistochemical and immunoblot analyses were used to investigate nasal cytochrome P-450 distribution in mice with either unilateral naris closure for 3, 4, or 5 months, or olfactory bulbectomy. P-450 immunoreactivity was observed only in the supporting cells and Bowman's glands of the olfactory mucosa. Immunoreactivity was clearly reduced in rostral regions of the open-side olfactory mucosa where losses of receptor neurons resulted from 3 to 5 months of closure. Closed-side immunoreactivity was similar to controls. In 4 month closure animals that had regrown their receptor neurons, open-side immunoreactivity was comparable to controls. Olfactory bulbectomy also depressed P-450 immunoreactivity. These data suggest that presence or absence of receptor neurons markedly affects P-450 expression in nonneuronal cells of the olfactory mucosa.
采用免疫组织化学和免疫印迹分析方法，研究单侧鼻孔封闭3、4或5个月的小鼠或嗅球切除术后小鼠鼻腔细胞色素P-450的分布情况。仅在嗅黏膜的支持细胞和鲍曼腺中观察到P-450免疫反应性。在开放侧嗅黏膜的前端区域，免疫反应性明显降低，在3至5个月的封闭过程中，该区域的受体神经元发生了损失。封闭侧的免疫反应性与对照组相似。在4个月封闭后受体神经元已再生的动物中，开放侧的免疫反应性与对照组相当。嗅球切除术也会降低P-450免疫反应性。这些数据表明，受体神经元的存在与否显著影响嗅黏膜非神经元细胞中P-450的表达。
