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[马约特岛和大科摩罗岛冈比亚按蚊复合体蚊子的分子特征分析]

[Molecular characterization of mosquitoes of the Anopheles gambiae complex from Mayotte and Great Comoro].

作者信息

Léong Pock Tsy J, Elissa N, Ouledi A, Ariey F, Duchemin J B, Robert V

机构信息

Groupe de recherche sur le paludisme, Institut Pasteur de Madagascar, BP 1274, Antananarivo 101, Madagascar.

出版信息

Parasite. 2003 Sep;10(3):273-6. doi: 10.1051/parasite/2003103273.

Abstract

The mosquitoes of the Anopheles gambiae complex have been characterised at specific and sub-specific levels in two islands of the Comoros archipelago: the island of Mayotte (French departmental collectivity) and the island of Grande Comore (Comoros Union). Results are similar in the two islands and are presented together. The species An. gambiae s.s. was observed alone (determination performed on 149 specimens by PCR product of IGS of rDNA). The molecular form observed alone was S, and corresponds in this geographic area to the chromosomal form Savanna (determination performed on 123 specimens by another PCR product of IGS of rDNA). The haplotype IB was observed alone (determination performed on ten specimens, by sequencing the ITS of rDNA, with special attention at the position 871 of ITS), as previously observed by other authors in East Africa. Finally, in Mayotte and Grande Comore the An. gambiae complex is only composed by An. gambiae s.s. from the molecular form S/type IB.

摘要

在科摩罗群岛的两个岛屿上,即马约特岛(法国海外省)和大科摩罗岛(科摩罗联盟),冈比亚按蚊复合体的蚊子已在种和亚种水平上得到鉴定。两个岛屿的结果相似,现将其合并呈现。单独观察到冈比亚按蚊指名亚种(通过rDNA的IGS的PCR产物对149个标本进行鉴定)。单独观察到的分子型为S型,在该地理区域对应于染色体型稀树草原(通过rDNA的IGS的另一个PCR产物对123个标本进行鉴定)。单独观察到单倍型IB(通过对rDNA的ITS进行测序,对10个标本进行鉴定,特别关注ITS的871位),正如其他作者先前在东非所观察到的那样。最后,在马约特岛和大科摩罗岛,冈比亚按蚊复合体仅由分子型S/单倍型IB的冈比亚按蚊指名亚种组成。

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