Micieli María V, García Juan J, Becnel James J
Comisión de Investigaciones Científicas Provincia Buenos Aires, Centro de Estudios Parasitológicos y de Vectores, Universidad Nacional de La Plata, Calle 2 No. 584, (1900) La Plata, Argentina.
Folia Parasitol (Praha). 2003 Sep;50(3):171-5.
A natural population of Psorophora ferox (Humbold, 1820) infected with the microsporidium Amblyospora ferocis Garcia et Becnel, 1994 was sampled weekly during a seven-month survey in Punta Lara, Buenos Aires Province, Argentina. The sequence of development of A. ferocis in larvae of P. ferox leading to the formation of meiospores followed the developmental pathway previously reported for various species of Amblyospora. The natural prevalence of A. ferocis in the larval population of P. ferox ranged from 0.4% to 13.8%. Spores were detected in the ovaries of field-collected females of P. ferox and were shown to be responsible for transovarial transmission of A. ferocis to the next generation of mosquito larvae in laboratory tests. These spores were binucleate and slightly pyriform in shape. The prevalence of A. ferocis in the adult population ranged from 2.7% to 13.9%. Data on effects of the infection on female fecundity showed that infected field-collected adults of P. ferox laid an average of 47.6 +/- 6.5 eggs of which 35.8% +/- 4.1% hatched. Uninfected field-collected adults of P. ferox laid 82.8 +/- 6.8 eggs of which 64.1% +/- 5.5% hatched. Six species of copepods living together with P. ferox were fed meiospores from field-infected larvae but none became infected. Horizontal transmission of A. ferocis to P. ferox larvae remains unknown.
在阿根廷布宜诺斯艾利斯省蓬塔拉拉进行的为期七个月的调查中，每周对感染了微孢子虫费氏安布罗孢子虫（Garcia和Becnel，1994年）的凶猛骚蚊自然种群进行采样。费氏安布罗孢子虫在凶猛骚蚊幼虫体内发育形成减数孢子的过程遵循了先前报道的各种安布罗孢子虫物种的发育途径。费氏安布罗孢子虫在凶猛骚蚊幼虫种群中的自然感染率为0.4%至13.8%。在野外采集的凶猛骚蚊雌性卵巢中检测到孢子，并且在实验室测试中表明这些孢子是费氏安布罗孢子虫经卵传递给下一代蚊虫幼虫的原因。这些孢子为双核，形状略呈梨形。费氏安布罗孢子虫在成虫种群中的感染率为2.7%至13.9%。关于感染对雌性繁殖力影响的数据表明，野外采集的感染了费氏安布罗孢子虫的凶猛骚蚊成虫平均产卵47.6±6.5枚，其中35.8%±4.1%孵化。未感染的野外采集的凶猛骚蚊成虫产卵82.8±6.8枚，其中64.1%±5.5%孵化。将与凶猛骚蚊生活在一起的六种桡足类动物喂食来自野外感染幼虫的减数孢子，但没有一种被感染。费氏安布罗孢子虫向凶猛骚蚊幼虫的水平传播情况仍然未知。
