Kateley J R, Nickerson D A, Gengozian N
Immunology. 1977 Nov;33(5):653-662.
Isotope (tritiated thymidine, [H]Tdr) incorporation into lymphocytes from two marmoset species, New World primates which are haemopoietic chimeras, was studied using peripheral blood lymphocyte (PBL) cultures incubated for 1–5 days with different concentrations of Concanavalin A (Con A), leucoagglutinin (LA), bacterial lipopolysaccharide (LPS) from gram-negative bacteria, rabbit anti-marmoset immunoglobulin (anti-Ig) serum, thymosin or irradiated histoincompatible (xenogeneic) lymphocytes. Only the plant lectins and xenogeneic lymphoid cells stimulated the uptake of [H]Tdr. Lymphocyte enrichment experiments demonstrated that cells responsive to plant lectins and xenoantigens were primarily thymus-derived (T) lymphocytes. Bacterial endotoxin (LPS), however, enhanced the mitogenic response of PBL to Con A when LPS and plant lectin were added together at culture initiation. Thymosin caused either enhancement or suppression of the response to Con A depending on its time of addition relative to lectin stimulation. Addition of thymosin to lymphocyte cultures with Con A or 24 h later caused a decrease in isotope incorporation, while addition of thymosin 48 h later caused a 12–100% increase in [H]Tdr uptake. Lymphocyte chimerism did not influence the mitogenic response since single-born, nonchimeric marmosets responded to plant lectins in a manner similar to marmosets with varying proportions of chimeric blood elements. Cytological analysis of stimulated lymphocytes from heterosexual marmosets revealed the percentage chimerism in the polyclonal mitogenic response and the clonal mixed lymphocyte reaction to be similar.
使用外周血淋巴细胞（PBL）培养物，研究了两种狨猴（新世界灵长类动物，造血嵌合体）淋巴细胞中同位素（氚标记胸腺嘧啶核苷，[H]Tdr）的掺入情况。将PBL培养物与不同浓度的伴刀豆球蛋白A（Con A）、白细胞凝集素（LA）、革兰氏阴性菌的细菌脂多糖（LPS）、兔抗狨猴免疫球蛋白（抗Ig）血清、胸腺素或经辐照的组织不相容（异种）淋巴细胞一起孵育1 - 5天。只有植物凝集素和异种淋巴细胞刺激了[H]Tdr的摄取。淋巴细胞富集实验表明，对植物凝集素和异种抗原产生反应的细胞主要是胸腺来源的（T）淋巴细胞。然而，当在培养开始时将细菌内毒素（LPS）和植物凝集素一起添加时，LPS增强了PBL对Con A的促有丝分裂反应。胸腺素对Con A反应的影响取决于其相对于凝集素刺激的添加时间。在含有Con A的淋巴细胞培养物中添加胸腺素或在24小时后添加会导致同位素掺入减少，而在48小时后添加胸腺素会使[H]Tdr摄取增加12% - 100%。淋巴细胞嵌合现象不影响促有丝分裂反应，因为单胎、非嵌合的狨猴对植物凝集素的反应方式与具有不同比例嵌合血细胞的狨猴相似。对异性狨猴刺激淋巴细胞进行的细胞学分析表明，多克隆促有丝分裂反应和克隆性混合淋巴细胞反应中的嵌合百分比相似。
