[枯草芽孢杆菌Rec突变体中SOS样系统的诱导]

[Induction of the SOS-like system in Rec-mutants of Bacillus subtilis].

作者信息

Suslov A V, Suslova I N, Kreneva R A, Kalinin V L

出版信息

Mol Gen Mikrobiol Virusol. 1992 May-Jun(5-6):16-9.

Abstract

Influence of the recE1, recB2, recB3, recB19, recF15, recF18, recL16, recM13 and recM27 mutations of the induction of the SOS-like system component, i. e. the RecE protein of Bacillus subtilis was studied by RIA-dot-blot method in UV-irradiated or treated by nalidixic acid cells. These agents caused a significant increase in the wild type (rec+) cells but did not stimulate the RecE synthesis in the rec mutants tested. The two exceptions were recB2 and recF18 mutants treated by nalidixic acid. The tsi23 mutation caused thermoinduction of phi 105 bacteriophage in the rec+ genetic background while no prophage particles were induced in the recE, recF, recL, recM mutants. The data suggest that the genetic damage of several rec genes including recB, recE, recF, recL and recM can block induction of the SOS-like system of Bacillus subtilis.

摘要

采用放射免疫斑点印迹法，研究了枯草芽孢杆菌recE1、recB2、recB3、recB19、recF15、recF18、recL16、recM13和recM27突变对紫外线照射或经萘啶酸处理的细胞中类SOS系统组分（即RecE蛋白）诱导的影响。这些因素使野生型（rec+）细胞中的RecE蛋白显著增加，但对所检测的rec突变体中的RecE合成没有刺激作用。两个例外是经萘啶酸处理的recB2和recF18突变体。tsi23突变在rec+遗传背景下导致φ105噬菌体的热诱导，而在recE、recF、recL、recM突变体中未诱导出前噬菌体颗粒。数据表明，包括recB、recE、recF、recL和recM在内的几个rec基因的遗传损伤可阻断枯草芽孢杆菌类SOS系统的诱导。