Peredel'chuk M Iu, Bukanov N O, Smirnov Iu V, Rostova Iu V, Fedorova N D, Okorokov A L, Gusiatiner M M, Iankovskiĭ N K
Mol Gen Mikrobiol Virusol. 1992 May-Jun(5-6):25-7.
Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.
携带来自谷氨酸棒杆菌的S-(2-氨基乙基)-L-半胱氨酸抗性突变菌株的天冬氨酸半醛脱氢酶（asd）基因的质粒，是通过在大肠杆菌中对asd突变进行互补，从构建在质粒克隆载体pSL5上的克隆文库中筛选出来的。已有证据表明，克隆的染色体DNA片段还包含反馈抗性天冬氨酸激酶lysC基因的完整序列。
