Gowthaman M K, Raghava Rao K S, Ghildyal N P, Karanth N G
Fermentation Technology and Bioengineering Discipline, Central Food Technological Research Institute, Mysore, India.
Biotechnol Adv. 1993;11(3):611-20. doi: 10.1016/0734-9750(93)90030-q.
In solid-state fermentation (SSF), interaction of heat and mass transfer with biochemical reaction (growth associated enzyme production) affects the bioreactor performance. This interaction was earlier observed to cause temperature and gaseous concentration gradients which reduced the effective bed height of the bioreactor. Since forced aeration is known to alleviate this problem, a packed column bioreactor with forced aeration was employed in the present study. Using wheat bran and Aspergillus niger CFTRI 1105, experiments were conducted for the production of the enzyme amyloglucosidase at various air flow rates. Temperatures and gas concentrations were recorded and enzyme activities estimated at different bed heights during the course of SSF. Gas concentration and temperature gradients decreased with increasing air flow rate. The packed column allowed the use of larger bed heights and yielded higher enzyme activities (6,260 Units/gDMB) than trays (345 Units/gDMB). Enzyme activity was affected more by temperature than concentration gradients, and increased with air flow rates.
在固态发酵（SSF）中，传热传质与生化反应（与生长相关的酶产生）之间的相互作用会影响生物反应器的性能。早期观察到这种相互作用会导致温度和气态浓度梯度，从而降低生物反应器的有效床层高度。由于已知强制通风可缓解此问题，因此在本研究中采用了带有强制通风的填充柱生物反应器。以麦麸和黑曲霉CFTRI 1105为原料，在不同空气流速下进行了生产糖化酶的实验。在固态发酵过程中，记录了不同床层高度处的温度和气体浓度，并估算了酶活性。气体浓度和温度梯度随空气流速的增加而降低。与托盘（345单位/克干物质）相比，填充柱允许使用更大的床层高度，并产生更高的酶活性（6260单位/克干物质）。酶活性受温度的影响比浓度梯度更大，并且随空气流速的增加而增加。
