通过基因工程改造的微生物细胞过量生产谷胱甘肽及其衍生物。

Overproduction of glutathione and its derivatives by genetically engineered microbial cells.

作者信息

Murata K, Kimura A

机构信息

Research Institute for Food Science, Kyoto University, Uji, Japan.

出版信息

Biotechnol Adv. 1990;8(1):59-96. doi: 10.1016/0734-9750(90)90005-v.

DOI:10.1016/0734-9750(90)90005-v

PMID:14545903

Abstract

In order to improve the biotechnological potentials of Escherichia coli cells to produce glutathione, S-D-lactoylglutathione and other gamma-glutamyl compounds, the genes for enzymes [gamma-L-glutamyl-L-cysteine synthetase (GSH A) in E. coli B, glutathione synthetase (GSH B) in E. coli B, glyoxalase I (GLO I) in Pseudomonas putida] were cloned and amplified in E. coli. E. coli B cells transformed with both GSH A and GSH B genes exhibited a high activity in the synthesis of glutathione and other gamma-glutamyl compounds in bioreactor systems containing immobilized cells. E. coli C600 cells transformed with GLO I gene of P. putida showed a high GLO I activity and were used for the preparation of S-D-lactoylglutathione and other glutathione thiol esters.

摘要

为了提高大肠杆菌细胞生产谷胱甘肽、S-D-乳酰谷胱甘肽及其他γ-谷氨酰化合物的生物技术潜力，将[大肠杆菌B中的γ-L-谷氨酰-L-半胱氨酸合成酶（GSH A）、大肠杆菌B中的谷胱甘肽合成酶（GSH B）、恶臭假单胞菌中的乙二醛酶I（GLO I）]的酶基因克隆并在大肠杆菌中扩增。用GSH A和GSH B基因转化的大肠杆菌B细胞在含有固定化细胞的生物反应器系统中合成谷胱甘肽及其他γ-谷氨酰化合物时表现出高活性。用恶臭假单胞菌的GLO I基因转化的大肠杆菌C600细胞表现出高GLO I活性，并用于制备S-D-乳酰谷胱甘肽及其他谷胱甘肽硫醇酯。