免疫亲和纯化：基本原理与操作注意事项

Immunoaffinity purification: basic principles and operational considerations.

作者信息

Yarmush M L, Weiss A M, Antonsen K P, Odde D J, Yarmush D M

机构信息

Department of Chemical and Biochemical Engineering and the Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08855, USA.

出版信息

Biotechnol Adv. 1992;10(3):413-46. doi: 10.1016/0734-9750(92)90303-q.

DOI:10.1016/0734-9750(92)90303-q

PMID:14546282

Abstract

Immunoaffinity purification has become an important technique in biotechnology. In this review the basic principles of immunoaffinity separations are described with respect to the stages of operation and potential application. The most commonly used support materials, activation procedures, and coupling chemistries are compared to one another for suitability in various applications. Individual operational steps for fixed bed immunoadsorbents including loading, washing, elution and regeneration are described in terms of both theory and practice. Factors influencing adsorbent stability are identified, and alternative operation and configuration strategies are discussed in light of their application to immunoaffinity systems.

摘要

免疫亲和纯化已成为生物技术领域的一项重要技术。在这篇综述中，将针对操作阶段和潜在应用来描述免疫亲和分离的基本原理。比较了最常用的载体材料、活化程序和偶联化学方法在各种应用中的适用性。从理论和实践两方面描述了固定床免疫吸附剂的各个操作步骤，包括上样、洗涤、洗脱和再生。确定了影响吸附剂稳定性的因素，并根据其在免疫亲和系统中的应用，讨论了替代操作和配置策略。