Kawagishi Aki, Kubosaki Atsutaka, Takeyama Natsumi, Sakudo Akikazu, Saeki Keiichi, Matsumoto Yoshitsugu, Hayashi Toshiharu, Onodera Takashi
Department of Molecular Immunology, School of Agricultural and Life Sciences, University of Tokyo, Japan.
Biochem Biophys Res Commun. 2003 Oct 24;310(3):791-5. doi: 10.1016/j.bbrc.2003.09.078.
Encephalomyocarditis (EMC) virus induces insulin-dependent diabetes and myocarditis in several strains of mice. The T-cell receptor (TCR) Vbeta genes of infiltrating T cells in the pancreas and myocardium of BALB/C mice infected with EMC virus D-variant (EMC-D virus) were analyzed. Using a nested two-step polymerase chain reaction (PCR), TCR Vbeta cDNAs were cloned and sequenced. Two and four kinds of TCR Vbeta clones were obtained from T cells infiltrating into the pancreas and myocardium of BALB/C mice infected with EMC-D virus, respectively. The infiltrating lymphocytes in the diabetic mice expressed Vbeta 8.1, 8.2, and 8.3 genes predominantly. Previously, the use of Vbeta 8.2 has been reported in autoimmune diseases such as murine experimental allergic encephalomyelitis (EAE) and non-obese diabetic (NOD) mouse. This study suggests that mice infected with EMC virus are a useful animal model for autoimmune diseases such as insulin-dependent diabetes.
脑心肌炎（EMC）病毒可在数种小鼠品系中诱发胰岛素依赖型糖尿病和心肌炎。对感染EMC病毒D变异株（EMC-D病毒）的BALB/C小鼠胰腺和心肌中浸润性T细胞的T细胞受体（TCR）Vβ基因进行了分析。采用巢式两步聚合酶链反应（PCR）对TCR Vβ cDNA进行克隆和测序。分别从感染EMC-D病毒的BALB/C小鼠浸润到胰腺和心肌的T细胞中获得了两种和四种TCR Vβ克隆。糖尿病小鼠中的浸润淋巴细胞主要表达Vβ 8.1、8.2和8.3基因。此前，在自身免疫性疾病如小鼠实验性变应性脑脊髓炎（EAE）和非肥胖糖尿病（NOD）小鼠中已报道过Vβ 8.2的使用情况。本研究表明，感染EMC病毒的小鼠是胰岛素依赖型糖尿病等自身免疫性疾病的有用动物模型。
