Bui B V, Fortune B, Cull G, Wang L, Cioffi G A
Discoveries In Sight, Legacy Clinical Research and Technology Center, Portland, OR 97208-3950, USA.
Exp Eye Res. 2003 Nov;77(5):555-66. doi: 10.1016/s0014-4835(03)00195-7.
This study assessed the inter-ocular and inter-session variability of the transient pattern electroretinogram (PERG) in a group of non-human primates. The transient PERG was measured both eyes of 29 non-human primates, and again after three months in 23 eyes of 23 of these animals. Signals were elicited using a contrast (90%, 75 cdm(-2)) reversing (5 reversals sec(-1)) checkerboard pattern (0.56 cpd). PERGs were also measured for stimuli of varied spatial frequency (n=8, 0.07-2.22 cpd), contrast (n=4, 20-100%), mean luminance (n=4, 4.7-75 cdm(-2)) and defocus (n=5, +1, +2, +3 diopters). The inter-eye and inter-session limits-of-agreement (LOA; 95%) were determined for each PERG parameter. Variability was also compared with previous studies using the coefficient-of-variability (COV). Pharmacological blockade of the inner retinal contributions to the PERG measured under these conditions was conducted in one animal using intravitreal injection of tetrodotoxin (approximately 6 microM) and N-methyl-D-aspartic acid (approximately 6 microM). The N95 component of the primate transient PERG showed spatial tuning, with a peak between 0.14 and 0.28cpd. This spatial tuning was not as apparent for the P50 component. A linear relationship between P50 and N95 amplitude was found with contrast and mean luminance. Both components were attenuated with the introduction of +2 diopters or more of defocus. The inter-session COV for the P50 and N95 components were 23.8 and 19.2%, respectively, while the LOA were 58 and 46%, respectively. The N95:P50 ratio had smaller inter-session variability, was robust to changes in contrast, mean luminance and defocus, and was effective for characterization of inner-retinal dysfunction after pharmacologic block.
本研究评估了一组非人类灵长类动物瞬态图形视网膜电图（PERG）的眼间和不同测量时段的变异性。对29只非人类灵长类动物的双眼进行了瞬态PERG测量，其中23只动物的23只眼睛在三个月后再次进行测量。使用对比度为90%（75 cd·m⁻²）、反转频率为5次/秒的棋盘格图案（0.56周/度）诱发信号。还对不同空间频率（n = 8，0.07 - 2.22周/度）、对比度（n = 4，20% - 100%）、平均亮度（n = 4，4.7 - 75 cd·m⁻²）和散焦（n = 5，+1、+2、+3屈光度）的刺激进行了PERG测量。确定了每个PERG参数的眼间和不同测量时段的一致性界限（LOA；95%）。还使用变异系数（COV）将变异性与先前的研究进行了比较。在一只动物中，通过玻璃体内注射河豚毒素（约6微摩尔）和N - 甲基 - D - 天冬氨酸（约6微摩尔），对这些条件下测量的PERG视网膜内层贡献进行了药理学阻断。灵长类动物瞬态PERG的N95成分表现出空间调谐，峰值在0.14至0.28周/度之间。这种空间调谐在P50成分中不那么明显。发现P50和N95振幅之间存在与对比度和平均亮度的线性关系。随着引入+2屈光度或更高的散焦，两个成分均减弱。P50和N95成分的不同测量时段COV分别为23.8%和19.2%，而LOA分别为58%和46%。N95:P50比值的不同测量时段变异性较小，对对比度、平均亮度和散焦的变化具有鲁棒性，并且在药理学阻断后对视网膜内层功能障碍的表征有效。
