Lee B Y, Choi S H, Ryu K H
Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University, Seoul, Republic of Korea.
Arch Virol. 2003 Oct;148(10):1915-24. doi: 10.1007/s00705-003-0161-2.
This study determined the 3'-terminal nucleotide sequences of two Korean isolates of Daphne virus S (DVS), a tentative member of the genus Carlavirus, causing leaf distortion and chlorotic spot disease symptoms in daphne plants. The 3'-terminal 1,465 nucleotide sequences of the two isolates contained two open reading frames coding for proteins of 36 kDa viral coat protein (CP) and 12 kDa from the 5'-3' end, which is a typical genome structure of the 3'-terminal region of carlaviruses. Both DVS isolates were 98.1% and 93.6% amino acid identical in the CP and 12 kDa, respectively. The CP gene of DVS shares 25.2-55.2% and 42.9-56.1% similarities with that of 19 other carlaviruses at the amino acid and nucleotide levels, respectively. The 3'-proximal 12 kDa gene of DVS shares 20.2-57.8% amino acid identities with that of 18 other members of the genus. The 3' noncoding region of DVS consists of 73 nucleotides with long excluding poly A tract, and shares 69.1-77.1% identities to the known carlaviruses. In the phylogenetic analyses of the two proteins, DVS was closely related to Helenium virus S and Chrysanthemum virus B. This is the first sequence information for the DVS, and further confirms the classification of DVS as a distinct member of the genus Carlavirus.
本研究确定了韩国的两种瑞香病毒S（DVS）分离株的3'末端核苷酸序列。DVS是麝香石竹潜隐病毒属的一个暂定成员，可在瑞香属植物中引起叶片扭曲和褪绿斑点病症状。这两种分离株的3'末端1465个核苷酸序列包含两个开放阅读框，从5'-3'端编码36 kDa病毒外壳蛋白（CP）和12 kDa的蛋白质，这是麝香石竹潜隐病毒属病毒3'末端区域的典型基因组结构。两种DVS分离株的CP和12 kDa蛋白的氨基酸同一性分别为98.1%和93.6%。DVS的CP基因在氨基酸水平和核苷酸水平上分别与其他19种麝香石竹潜隐病毒的CP基因有25.2 - 55.2%和42.9 - 56.1%的相似性。DVS的3'近端12 kDa基因与该属其他18个成员的基因有20.2 - 57.8%的氨基酸同一性。DVS的3'非编码区由73个核苷酸组成，不包括长的聚腺苷酸序列，与已知的麝香石竹潜隐病毒有69.1 - 77.1%的同一性。在对这两种蛋白质的系统发育分析中，DVS与向日葵病毒S和菊花病毒B密切相关。这是关于DVS的首个序列信息，并进一步证实了DVS作为麝香石竹潜隐病毒属一个独特成员的分类。
