通过类核区封闭定位球形大肠杆菌中的细胞分裂

Localizing cell division in spherical Escherichia coli by nucleoid occlusion.

作者信息

Zaritsky Arieh, Woldringh Conrad L

机构信息

Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel.

出版信息

FEMS Microbiol Lett. 2003 Sep 26;226(2):209-14. doi: 10.1016/S0378-1097(03)00580-9.

DOI:10.1016/S0378-1097(03)00580-9

PMID:14553913

Abstract

The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes ('steps') in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015-1022]. In such cells, fluorescent FtsZ-GFP arcs did not overlap the DAPI-stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030-6038].

摘要

通过短暂暴露于美西林使大肠杆菌thyA细胞呈球形，并利用胸腺嘧啶浓度的变化（“步移”）来操纵染色体复制时间，在此条件下研究了FtsZ定位与类核分离之间的空间关系[扎里茨基等人，《微生物学》145（1999）1015 - 1022]。在这类细胞中，荧光FtsZ - GFP弧不与经DAPI染色的类核重叠。得出的结论是，FtsZ环沉积在正在分离的类核之间，这与类核阻隔模型相符[沃尔德林等人，《细菌学杂志》176（1994）6030 - 6038]。