Yu Yan-chun, Chu Ying, Ji Jian-Yie
Department of Chemical Engineering, Huaihai Institute of Technology, Lanyungang 222005, People's Republic of China.
J Colloid Interface Sci. 2003 Nov 1;267(1):60-4. doi: 10.1016/s0021-9797(03)00450-8.
The experimental results of yeast-lipase extracted by AOT-reverse-micelles in isooctane are reported. The effects of pH values and ionic strength in the aqueous phase, surfactant concentration, phase volume ratio, temperature, stirring time, and cosolvent concentration on the phase transfer of the lipase by the reverse micelles are studied. One hundred percent of forward extraction yield, 68% of back extraction yield, and 45% of activity recovery yield for the lipase are obtained. The phase transfer of the lipase is controlled by electrostatic and hydrophobic interactions.
报道了用AOT反胶束在异辛烷中提取酵母脂肪酶的实验结果。研究了水相中的pH值、离子强度、表面活性剂浓度、相体积比、温度、搅拌时间和助溶剂浓度对脂肪酶通过反胶束进行相转移的影响。脂肪酶的正向萃取率为100%，反向萃取率为68%，活性回收率为45%。脂肪酶的相转移受静电和疏水相互作用控制。
