猫泡沫病毒基因组与复制策略。
Feline foamy virus genome and replication strategy.
作者信息
Roy Jacqueline, Rudolph Wolfram, Juretzek Thomas, Gärtner Kathleen, Bock Michael, Herchenröder Ottmar, Lindemann Dirk, Heinkelein Martin, Rethwilm Axel
机构信息
Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
出版信息
J Virol. 2003 Nov;77(21):11324-31. doi: 10.1128/jvi.77.21.11324-11331.2003.
Crucial aspects of the foamy virus (FV) replication strategy have so far only been investigated for the prototypic FV (PFV) isolate, which is supposed to be derived from nonhuman primates. To study whether the unusual features of this replication pathway also apply to more-distantly related FVs, we constructed feline FV (FFV) infectious molecular clones and vectors. It is shown by quantitative RNA and DNA PCR analysis that FFV virions contain more RNA than DNA. Full-length linear DNA was found in extracellular FFV by Southern blot analysis. Similar to PFV, azidothymidine inhibition experiments and the transfection of nucleic acids extracted from extracellular FFV indicated that DNA is the functional relevant FFV genome. Unlike PFV, no evidence was found indicating that FFV recycles its DNA into the nucleus.
迄今为止，泡沫病毒（FV）复制策略的关键方面仅针对原型FV（PFV）分离株进行了研究，该分离株被认为源自非人类灵长类动物。为了研究这种复制途径的异常特征是否也适用于亲缘关系更远的FV，我们构建了猫泡沫病毒（FFV）感染性分子克隆和载体。通过定量RNA和DNA PCR分析表明，FFV病毒粒子含有的RNA比DNA多。通过Southern印迹分析在细胞外FFV中发现了全长线性DNA。与PFV相似，叠氮胸苷抑制实验以及对从细胞外FFV中提取的核酸进行转染表明，DNA是与FFV功能相关的基因组。与PFV不同，没有发现证据表明FFV将其DNA循环回细胞核。
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